Cannabinoid Methodology

  • Cannabinoid Methodology

    For those preferring a specific method for their analytical testing needs, Anresco Laboratories offers potency profiling utilizing 2 different instruments, the pros and cons of which are explained below:

    Liquid Chromatography Ultraviolet (LC-UV)

    Recommended by The American Herbal Pharmacopoeia (AHP), LC-UV will be the industry standard for cannabinoid testing performed to comply with California state law in 2018. Using liquid chromatography (LC), the sample (in liquid form) is injected at high pressure through a column composed of irregularly shaped particles. With each compound traveling at a different speed, the chemical components are separated and the output is directed into a UV detector that is able to identify each cannabinoid. Using this technique, Anresco has the capability to quantify the presence of 11 cannabinoids: ∆9-THC, ∆8-THC, THCA, THCV, CBD, CBDA, CBDV, CBG, CBGA, CBN, and CBC.

    Gas Chromatography with Flame Ionization Detector (GC-FID)

    Another commonly utilized method, GC-FID uses a hydrogen/air flame to oxidize the organic molecules from the sample and produce electrically charged particles (ions). These ions are then collected on a plate and measured based on their electric current. Using this technique, Anresco has the capability to quantify the presence of 6 cannabinoids: ∆9-THC, ∆8-THC, CBD, CBG, CBN, CBC.

    Although well-regarded in the analytical industry, GC-FID incidentally converts the acidic cannabinoids into their active counterparts in a process called “decarboxylation” due to the high temperature in which the sample is vaporized. Resultantly, only the total content of each cannabinoid can be computed and thus, this method will not comply with California state regulations once implemented.